Tozer hailed from a tiny farming community in western La Jose, Pennsylvania. He was converted to Christianity as a teenager in Akron, Ohio: While on his way home from work at a tire company, he overheard a street preacher say, “If you don’t know how to be saved … just call on God , saying, ‘Lord, be merciful to me a sinner.'” Upon returning home, he climbed into the attic and heeded the preacher’s advice.
In 1919, five years after his conversion and without formal education in Christian theology, Tozer accepted an offer to serve as pastor of his first church. That began 44 years of ministry associated with the Christian and Missionary Alliance (C&MA), a Protestant Evangelical denomination, of them serving as a pastor in several different congregations (his first, a small storefront church in Nutter Fort, West Virginia). Later, he served thirty years (1928 to 1959) as the pastor of Southside Alliance Church in Chicago; the final years of his life he spent as pastor of Avenue Road Church in Toronto, Ontario, Canada. Observing contemporary Christian living, Tozer felt that the church was on a dangerous course toward compromising with “worldly” concerns.

Born into poverty, Tozer was self-educated and taught himself what he missed in high school and university.